The RM016 3-color Pre-Stained Protein Marker is a ready to use three-color protein standard with 13 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine buffer (3.5 to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with two reference proteins carrying enhanced intensity corresponding to a green at 25 kDa and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer).
Designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
5 μl per well for 100 applications
Contents | Approximately 0.2~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5), 2% SDS, 1 mM DTT, 4.8 M urea, and 12% (v/v) glycerol). |
Quality Control | Under suggested conditions, RM016 Pink Blue Protein Marker resolves 11 major bands in 15% SDS-PAGE (Tris-Glycine Buffer) and after Western blotting to nitrocellulose membrane. |
Loading Volume | 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively;1.5~2.5 μl per well for general Western transferring;Apply more for thicker (> 1.5 mm) or larger gel. |
Storage | 4°C ≥ 3 months; -20°C ≥ 24 months |
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