概述

Based on the rigid Agarose 6 Fast Flow matrix, with human IgG covalently coupled to it. The improved mechanical characteristics of this fast flow medium allows high flow rates to be used for rapid and convenient single step purification of protein A fusion protein conjungates produced in prokaryotic expression systems, such as the phagemid pEZZ 18 contains the protein A signal sequence and two synthetic “Z” domains based on the “B” IgG binding domain of protein A. Proteins are expressed as fusions with the “ZZ” peptide and secreted into the aqueous culture medium under the direction of the protein A signal sequence. They are easily purified using IgG Resin to which the “ZZ” domain binds tightly. Because of its unique folding properties, the 14 kDa “ZZ” peptide has little effect on folding of the fusion partner into a native conformation.

特点

• Feature:Binding capacity:> 2 mg protein A/ml resin. Ligand concentration 6~10 mg human IgG/ml resin, supplied as a slurry in 20% ethanol. .