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此重力柱是以兰色琼脂糖为亲和填料，该填料用sepharose 6 FF与Cibacron Blue F3G-A通过三嗪基相连，由于它有着与NAD+ and NADP+之类的辅助因子有着相似的结构，所以可以用亲和纯化一些激酶、脱氢酶和一些需要腺苷作为辅助因子的酶。通常用于albumin、interferon、a broad range of nucleotide requiring enzymes、α2-macroglobulin、coagulation factors和 nucleic acid binding proteins等的纯化，具有较高的稳定性和较好的再生性能。
| Resin volume | 1 ml/column |
| Ligand | Cibacron Blue F3G-A |
| Ligand concentration | 3 mg/ml gel or 5 umol cibacron blue F3G-A/ml drained gel |
| Binding capacity | 10 mg HSA/ml gel Particle size range 45~165 μm |
| Bead structure | Highly cross-linked spherical a |
| garose | 0.06 |
| Max flow rate | 4 ml/min (1 ml) |
| Recommended flow rate | 1 ml/min (1 ml) |
| pH stability | 4~12 (long time), 3~13 (short time) |
| Temperature stability | +4 °C to room temperature |
| Storage buffer | 20% ethanol |
| Compatible reagents | 8M urea, 6M guanidine hydrochloride and so on |
| Imcompatible reagents | Tween-20 and similar detergent in binding buffer, which will reduce binding capacity for albumin |
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