概述

SG-Chymotrypsin? is a serine endopeptidase, which predominantly cleaves peptide bonds on the carboxy side of tyrosine, phenylalanine and tryptophan. In addition, chymotrypsin has a low catalytic activity against the carboxy side of leucine, methionine, alanine, aspartic and glutamic acids. It is therefore recommended to always use the shortest digestion time possible. SG-Chymotrypsin? is first treated with TLCK to inhibit trypsin that may be present and then subjected to an extensive purification process to remove contaminating protease and chymotryptic autolysis by-products. The highly purified enzyme is then chemically modified to increase its resistance to autolysis and stability.For protein digestion, SG-Chymotrypsin? is added to the protein at a ratio of 1:200 to 1:50, by weight, in a standard digestion buffer. Incubate at 25~30°,C for 1 to 10 hours, but can be extended to 24 hours, due to the extended life of the SG-Chymotrypsin?. We recommend choosing a ratio of enzyme to protein that allows for the shortest incubation time possible. This will reduce or eliminate the catalyzed hydrolysis of peptide bonds with non-aromatic amino acid residues.

特点

• Modified Chymotrypsin for sequence analysis. Resistant to autolysis and degradation,
• High Specificity, free from other known endopeptidases. Consistency of activity levels from lot to lot for reproducible digestions,
• Recommended SG-Chymotrypsin? to protein ratio, 1:200 to 1:50.

应用

Application for digestion of proteins for sequence analysis and suitable for sequencing applications.

组份

SG‐Chymotrypsin?, Digestion Buffer (CHY)