SG-Arginine-C? endopeptidase (Clostripain, from C. histolyticum) specifically hydrolyzes the carboxy peptide bond of Arginine and has been modified chemically by a propriety process to render the enzyme resistant to autolysis and stabilize enzymatic activity. In addition, as a sulfhydryl enzyme, SG-Arginine-C? is susceptible to inactivation by oxidation and as a result requires reducing agents for protection. The enzyme also requires calcium ion for maximal activity. A special reconstitution buffer is supplied, which contains reducing agents and activators to maintain enzyme activity.SG-Arginine-C? is supplied lyophilized in an activated form in 5 μg vials and can be reconstituted to a concentration of 0.25 μg/ml by addition of 20 μl per vial of the supplied reaction buffer. For fragmentation, the enzyme is added to the protein sample in a ratio of 1:100 to 1:20 (enzyme to protein, by weight).
Application for digestion of proteins for sequence analysis and suitable for sequencing applications.
SG‐Arginine‐C?, Digestion Buffer (ARG)
温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知,且每位咨询者购买情况、提问时间等不同,为此以下回复仅对提问者3天内有效,其他网友仅供参考!若由此给您带来不便请多多谅解,谢谢!
服务热线
0771-3293894