概述

Biotin, a 244 Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=1015 M-1). Biotin and avidin interaction is rapid and once the bond is established it can survive up to 3M guanidine-hydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH1.5 or by boiling in SDS Page Sample Loading Buffer. Avidin is a glycoprotein with approximately 10% of its total mass coming from carbohydrates. Avidin has a molecular weight of 67 kDa and contains four identical 128 amino acid subunits that each has a single biotin binding domain. Avidin is a basic protein with an isoelectric pH of 10~10.5 and is readily soluble in aqueous buffers containing a wide range of salt, pH (2~11), temperature and other laboratory agents. This wide range of tolerance makes avidin suitable for a wide variety of analytical applications. Avidin has extraordinary binding affinity for biotin (Ka=1015 M-1). The avidin in covalently coupled to the agarose resulting in minimal leaching and is stable over pH2~11. The Avidin Resin is designed for the single step small and large scale affinity purification of proteins and antibodies with a biotin tag. The resin can also be used for immunoprecipitations using biotin labeled antibodies. Supplied as a 50% resin slurry.

特点

• Avidin covalently coupled to ~6% cross linked agarose. Minimal Leaching.Binding capacity 15~20 μg biotin/ml resin. For immunoprecipitation with biotinylated antibodies. Pull down assays with biotinylated proteins. Purification of biotinylated molecules, including:proteins, antibodies, DNA, carbohydrates.

应用

For immunoprecipitation with biotinylated antibodies. Pull down assays with biotinylated proteins. Purification of biotinylated molecules, including: proteins, antibodies, DNA, carbohydrates.

技术规格