概述

Streptavidin is a tetrameric protein containing 4 biotin binding sites. In many respects, streptavidin is similar to avidin except that it has no carbohydrate and has a slightly lower molecular weight of about 60 kDa. The solubility of streptavidin (isoelectric pH5) in aqueous buffer is much lower than avidin, but the binding of streptavidin to biotin is similar to that of avidin. The advantage of streptavidin is that the lack of carbohydrates significantly reduces the amount of non-specific binding. The streptavidin used for immobilization on porous 6% cross-linked agarose is a recombinant form, with a mass of 53 kDa and a near neutral pH. The streptavidin is covalently coupled to the agarose, resulting in minimal leaching, and is stable over a pH range of 2 to 11, The Steptavidin Resin is designed for the single step affinity purification of proteins and antibodies and other molecules with a biotin tag, furthermore be used for assay development and immunopreciptations of proteins using biotin labeled antibodies.

应用

Application for immunoprecipitation with biotinylated antibodies; Pull down assays with biotinylated proteins; Purification of biotinylated molecules including proteins, antibodies, DNA, carbohydrates.

技术规格