概述

Fraction-FOCUS? utilizes proven technology to fractionate and concentrate all proteomes into multiple fractions; simplifying 2D maps and enhancing detection of low abundant proteins.Fraction-FOCUS? is fully compatible with all downstream protein identification techniques.At the end of the fractionation, cellular proteins are in one of many fractions. The entire fractionation is carried out in micro scale.

特点

• Serial fractionation of protein samples into multiple protein fractions;
• Resulting fractions are fully compatible with 2D electrophoresis or isoelectric focusing and other applications;
• Application for fractionation of soluble proteins for cleaner 2D gels maps;
• Suitable for electrophoresis and other applications.

应用

The Fraction-FOCUS? kit is designed for 10 preparations.One assay for:100mg mammalian tissue、50μl wet animal cell pellet、50μl wet yeast pellet、50μl wet bacteria pellet、250mg plant tissue;^Fractionation of soluble proteins for cleaner 2D gels maps;Suitable for electrophoresis and other applications.

组份

Soluble Protein Extraction Buffer (SPE Buffer); Fraction Precipitation Buffer (FP Buffer); Universal Protein Precipitation Agent-I (UPPA-I); Universal Protein Precipitation Agent-II (UPPA-II); Orgosol? Buffer; FOCUS? Protein Solubilization Buffer (FPS Buffer); DILUENT-III; 2ml Tubes