概述

The Protease Assay Kit is designed for the quantitative determination of proteases present in a protein sample, using a dye-labeled protein substrate. The proteases present in the sample of interest will digest the protein substrate and release dye labeled peptides. The absorbance of the dye-labeled peptide is measured at 570 nm for determination of protease activity. Chemically stabilized Trypsin (MSG-Trypsin?) is supplied with the kit as a general protease standard; however, other specific protease standards can also be used. MSG-Trypsin? is an ultra-pure trypsin from bovine pancreas, modified by methylation followed by TPCK treatment and is extremely resistant to autolysis. The kit components are sufficient for 50 assays in a microtiter plate format or 0.5 ml assay tubes. Application for determination of protease activity in biological samples, with nanogram detection levels.

组份

Incubation Buffer; Precipitation Agent; Assay Buffer; Protease Substrate (lyophilized); MSG-Trypsin, porcine; Trypsin Suspension Buffer