概述

A fast and efficient spin column assay. Add the protein sample to the spin columns and wash to remove non-protein agents, including detergents and chaotropes. Next, add the protein dye and spin to remove free dye. After a second brief wash, elute the protein bound dye with the supplied elution buffer and measure the optical density of the dye. The concentration of protein is determined by comparing the optical density data to the supplied reference data. No protein standards are required. Protein concentration can be determined in less than 10 minutes.

特点

• Linear response in the range of 0.5 μg to 10 μg; Manual, Semi-automatic or fully automated compatible;
• Small sample requirement; Unaffected by non-protein chemicals and agents;
• Rapid assay; protein standards not required.

组份

SPN Column; SPN Assay Dye; SPN Wash Buffer-I;SPN Wash Buffer-II; SPN Elution Buffer; SPN Tables I & II: Lot Specific (with / without SDS)