The 2xTaqMan Fast qPCR Master Mix(High Rox) is a ready-to-use cocktail containing fast hot start Taq DNA Polymerase, MgSO4, dNTP Mix and some stabilizers. It is supplied as a 2X Master Mix containing all components except primers, probe and template for fast-cycling probe-based real-time PCR. The fast hot start Taq DNA Polymerase and proprietary buffer system reduce the formation of the primer dimers, enhance the amplification specificity and high detection sensitivity in real-time qPCR. The DNF Buffer is specific for the primers which exist multi nonspecific amplification. For some DNA sequences there are no good primers, the DNF Buffer can be added to the reaction mix according to the following protocol. The ROX reference dye is 1 μM in the 2xTaqMan Fast qPCR Master Mix(High Rox) and applicable to the real time PCR instruments that are need to normalization by the high concentration of Rox reference, like Applied Biosystems 5700, Applied Biosystems 7000, Applied Biosystems 7300, Applied Biosystems 7700, Applied Biosystems 7900, Applied Biosystems 7900HT, Applied Biosystems 7900 HT Fast, Applied Biosystems StepOne? and Applied Biosystems StepOnePlus?.
基因表达分析、拷贝数分析
2xTaqMan Fast qPCR Master Mix(High Rox); DNF Buffer; Sterilized ddH2O.
温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知,且每位咨询者购买情况、提问时间等不同,为此以下回复仅对提问者3天内有效,其他网友仅供参考!若由此给您带来不便请多多谅解,谢谢!
服务热线
0771-3293894