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| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Recognition sites | C↑CGC |
| Source | Bacillus species AC |
| Assayed on | Lambda DNA |
| Optimal buffer | 50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT + BSA |
| Optimal temperature | 37°C |
| Storage conditions | 10 mM KH2PO4(pH 7.2); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C. |
| Ligations | After 5-fold overdigestion with enzyme more than 95% of the Lambda DNA fragments can be ligated with T4 DNA Ligase at 16°C and 50% of these can be recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. |
| Methlation effect | Blocked by CG methylation. |
| Thermal inactivation | enzyme is inactivated by incubation at 65°C for 20 mins. |
| Campatible Ends | AciI, BspACI |
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