Unit definition | One unit is the amount of enzyme that will dephosphorylate 1 μg of pUC19 DNA (linearized with Hind III) in 30 minutes at 25°C. Dephosphorylation is defined as >95% inhibition of recirculation in a self-ligation reaction that is measured by transformation into E.coli. |
Source | Isolated from E.coli strain that carries the cloned Alkaline Phosphatase gene from Alteromonas undina P2 |
Optimal buffer | SE-buffer W, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) |
Optimal temperature | 25°C |
Storage conditions | 20 mM Tris-HCl (pH 7.6), 0.1 mM ZnCl2, 50% glycerol. Store at -20°C |
Thermal inactivation | enzyme is inactivated by incubation at 65°C for 20 mins. |
温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知,且每位咨询者购买情况、提问时间等不同,为此以下回复仅对提问者3天内有效,其他网友仅供参考!若由此给您带来不便请多多谅解,谢谢!
服务热线
0771-3293894