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| Unit definition | One unit is the amount of enzyme that will dephosphorylate 1 μg of pUC19 DNA (linearized with Hind III) in 30 minutes at 25°C. Dephosphorylation is defined as >95% inhibition of recirculation in a self-ligation reaction that is measured by transformation into E.coli. |
| Source | Isolated from E.coli strain that carries the cloned Alkaline Phosphatase gene from Alteromonas undina P2 |
| Optimal buffer | SE-buffer W, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) |
| Optimal temperature | 25°C |
| Storage conditions | 20 mM Tris-HCl (pH 7.6), 0.1 mM ZnCl2, 50% glycerol. Store at -20°C |
| Thermal inactivation | enzyme is inactivated by incubation at 65°C for 20 mins. |
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