Enzyme is active in presence of EDTA.
Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl. |
Recognition sites | ACTGGG(N)5↑ |
Source | Bacillus megaterium S87 |
Assayed on | Lambda DNA |
Optimal buffer | Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) |
Optimal temperature | 37°C |
Storage conditions | 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C |
Ligations | After 2-fold overdigestion with enzyme about 75% of the DNA fragments can be ligated and 95% may be recut. |
Non-specific hydrolisis | Overnight digest with BmuI is not recommended. A 50 μl reaction containing 1 μg of Lambda DNA and 0.5 units of enzyme incubated for 4 hours resulted in the same pattern of DNA bands as a reaction incubated for 1 hour. |
Methlation effect | Methylation sensitivity not tested |
Thermal inactivation | enzyme is inactivated by incubation at 65°C for 20 mins. |
Campatible Ends | BfiI , BmrI |
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