0
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Recognition sites | GAAGA(N)8↑ |
| Source | E.coli strain, that carries the cloned gene Mbo II from Moraxella bovis |
| Assayed on | Lambda DNA (dam-) |
| Optimal buffer | Y?(33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) |
| Optimal temperature | 37°C |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C. |
| Ligations | After 5-fold overdigestion with enzyme more than 60% of the DNA fragments can be ligated and recut. In presence of 10%PEG ligation is better. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C. |
| Methlation effect | Blocked by overlappin dam-methylation(GmATC):?GAAGATC. |
| Thermal inactivation | enzyme is inactivated by incubation at 65°C for 20 mins. |
温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知，且每位咨询者购买情况、提问时间等不同，为此以下回复仅对提问者3天内有效，其他网友仅供参考！若由此给您带来不便请多多谅解，谢谢！
服务热线
0771-3293894
新浪微博
腾讯微博
QQ空间
客服
