Unit definition | One unit is defined as the amoumt of enzyme required to give 50% ligation of Hind III fragments of Lambda DNA (5 DNA termini concentration of 0.12 μM [300 μg/ml]) in 20 ul of 1 x T4 DNA Ligase Reaction Buffer in 30 minutes at 16°C. ATP is an essential cofactor for the reaction |
Source | Isolated from E.coli strain that carries the cloned DNA ligase gene from bacteriophage T4 |
Optimal buffer | SE-buffer DNA ligase T4, (50 mM Tris-HCl (pH 7.8 at 25°C); 10 mM MgCl2; 10 mM DTT; 1 mM ATP.) |
Optimal temperature | 16°C |
Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C. |
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