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| Unit definition | One unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from double-stranded, uracil-containing DNA. Activity is measured by release of [3H]-uracil in a 50 ul reaction containing 0, 2 μg DNA (104?~ 105?cpm/μg) in 30 minutes at 37°C. |
| Source | Isolated from the recombinant source |
| Optimal buffer | SE-buffer Uracil DNA Glycosylase, (20 mM Tris-HCl (pH 8.0 at 25°C); 1 mM EDTA; 1 mM DTT) |
| Optimal temperature | 37°C |
| Storage conditions | 10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20°C. |
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