0
At 37°C activity is 10% from maximum.
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of pBR322 DNA in 1 hour at 55°C in a total reaction volume of 50 μl. |
| Recognition sites | GCAGC(N)8↑ |
| Source | Bacillus stearothermophilus V1 |
| Assayed on | pBR322 DNA |
| Optimal buffer | G?(10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) |
| Optimal temperature | 55°C |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C. |
| Ligations | After 3-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 55°C. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 80°C for 20 mins. |
| Campatible Ends | BbvI, BseXI, Bst71I, R9896 |
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