High enzyme concentration and incubation at 65°C for 16 hours results in star activity.
Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl. |
Recognition sites | GACNN↑NNGTC |
Source | Bacillus stearothermophillus PA |
Assayed on | Lambda DNA |
Optimal buffer | Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) |
Optimal temperature | 65°C |
Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C. |
Ligations | After 5-fold overdigestion with enzyme less than 5% of the DNA fragments can be ligated |
Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 25°C |
Methlation effect | Methylation sensitivity not tested |
Thermal inactivation | NO |
Campatible Ends | BoxI, PshAI |
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