Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl. |
Recognition sites | T↑TAA |
Source | E.coli strain that carries the cloned Tru9 I gene from Thermus ruber 9 |
Assayed on | Lambda DNA |
Optimal buffer | W?(10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) |
Optimal temperature | 65°C |
Storage conditions | 10 mM Tris-HCl-(pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol Store at -20°C. |
Ligations | After 20-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut. |
Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 65°C. |
Methlation effect | Blocked by TTAmA methylation . |
Thermal inactivation | enzyme is inactivated by incubation at 80°C for 20 mins. |
Campatible Ends | MseI, SaqAI, Tru1I |
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