To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.?
Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl. |
Recognition sites | C↑TRYAG |
Source | Bacillus stearothermophilus SF |
Assayed on | Lambda DNA |
Optimal buffer | O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA |
Optimal temperature | 60°C |
Storage conditions | 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol; Store at -20°C. |
Ligations | After 3-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut. |
Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 60°C. |
Methlation effect | Methylation sensitivity not tested |
Thermal inactivation | NO |
Campatible Ends | BfmI , BpcI, SfcI |
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