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| Sources | Recombinant E. coli strain that carries the cloned eagIR gene from Enterobacter agglomerans. |
| Storage conditions | 10 mM Tris-HCl (pH 8.2 at 25°C), 500 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/mL BSA, 50% glycerol. |
| Optimal buffer(10X) | 100 mM Tris-HCl (pH 8.5), 30 mM MgCl2, 1000 mM NaCl, 1 mg/mL BSA. |
| Unit Definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Assayed on | Lambda DNA |
| Optimal temperature | 37°C |
| Ligations | After 10-fold over digestion with enzyme 95% of the DNA fragments can be ligated and recut. |
| Methylation Effects: | Not dam methylation-sensitive, not dcm methylation- sensitive. CpG methylation-sensitive |
| Thermal Inactivation: | Enzyme is inactivated by incubation at 65°C for 20 minutes. |
| Campatible Ends | Bsp120 I, Cfr I, Not I. |
| Recognition sites | C↑GGCCG |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. |
| Sources | Recombinant E. coli strain that carries the cloned eagIR gene from Enterobacter agglomerans. |
| Storage conditions | 10 mM Tris-HCl (pH 8.2 at 25°C), 500 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/mL BSA, 50% glycerol. |
| Optimal buffer(10×) | 100 mM Tris-HCl (pH 8.5), 30 mM MgCl2, 1000 mM NaCl, 1 mg/mL BSA. |
| Unit Definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Assayed on | Lambda DNA |
| Optimal temperature | 37°C |
| Ligations | After 10-fold over digestion with enzyme 95% of the DNA fragments can be ligated and recut. |
| Methylation Effects: | Not dam methylation-sensitive, not dcm methylation- sensitive. CpG methylation-sensitive |
| Thermal Inactivation: | Enzyme is inactivated by incubation at 65°C for 20 minutes. |
| Campatible Ends | Bsp120 I, Cfr I, Not I. |
| Recognition sites | C↑GGCCG |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. |
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