Sources | Recombinant E. coli strain that carries the cloned apaLIR gene from Acetobacter pasteurianus. |
Storage conditions | 10 mM Tris-HCl (pH 7.5 at 25°C), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.2 mg/mL BSA and 50% glycerol. |
Optimal buffer(10X) | 330 mM Tris-acetate (pH 7.9), 100 mM magnesium acetate, 660 mM potassium acetate, 1 mg/mL BSA. |
Unit Definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
Assayed on | Lambda DNA |
Optimal temperature | 37°C |
Ligations | After 10-fold over digestion with enzyme 95% of the DNA fragments can be ligated and recut. |
Methylation Effects: | Not dam methylation-sensitive, CpG methylation-sensitive, Not dcm methylation-sensitive. |
Thermal Inactivation: | Enzyme is inactivated by incubation at 65°C for 20 minutes. |
Campatible Ends | Bfm I |
Recognition sites | G↑TGCAC |
Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. |
Sources | Recombinant E. coli strain that carries the cloned apaLIR gene from Acetobacter pasteurianus. |
Storage conditions | 10 mM Tris-HCl (pH 7.5 at 25°C), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.2 mg/mL BSA and 50% glycerol. |
Optimal buffer(10×) | 330 mM Tris-acetate (pH 7.9), 100 mM magnesium acetate, 660 mM potassium acetate, 1 mg/mL BSA. |
Unit Definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
Assayed on | Lambda DNA |
Optimal temperature | 37°C |
Ligations | After 10-fold over digestion with enzyme 95% of the DNA fragments can be ligated and recut. |
Methylation Effects: | Not dam methylation-sensitive, CpG methylation-sensitive, Not dcm methylation-sensitive. |
Thermal Inactivation: | Enzyme is inactivated by incubation at 65°C for 20 minutes. |
Campatible Ends | Bfm I |
Recognition sites | G↑TGCAC |
Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. |
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