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To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.?
| Sources | Recombinant E. coli strain that carries the cloned avaIIIR gene from Anabaena variabilis. |
| Storage conditions | 10 mM Tris-HCl (pH 7.5 at 25°C), 100 mM KCl, 10 mM MgCl2, 0.1 mM EDTA, 1 mM DTT, 0.01% BSA, 50% (v/v) glycerol. |
| Optimal buffer(10X) | 100 mM Tris-HCl (pH 7.5), 100 mM MgCl2, 500 mM NaCl, 1 mg/ml BSA. |
| Unit Definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Campatible Ends | BfrBl, EcoT22I, Mph1103I, NsiI, Zsp2I |
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