To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
Storage conditions | 10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/mL BSA, 50% glycerol. |
Optimal buffer(10X) | 330 mM Tris-acetate (pH 7.9), 100 mM magnesium acetate, 660 mM potassium acetate, 1 mg/mL BSA |
Unit Definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
Assayed on | Lambda DNA |
Ligations | After 10-fold over digestion with enzyme 90% of the DNA fragments can be ligated and recut |
Methylation Effects: | Not CpG methylation-sensitive, Not dcm methylation-sensitive, dam methylation-sensitive |
Thermal Inactivation: | Enzyme is inactivated by incubation at 65°C for 20 minutes |
Campatible Ends | Avr I, Bcu I, Eco130 I, Nhe I, Spe I, XmaJ I. |
温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知,且每位咨询者购买情况、提问时间等不同,为此以下回复仅对提问者3天内有效,其他网友仅供参考!若由此给您带来不便请多多谅解,谢谢!
服务热线
0771-3293894