0
At 37°C activity is about 70% from maximum.
| Sources | Recombinant E. coli strain that carries the cloned xhoIR gene from Xanthomonas holcicola. |
| Storage conditions | 10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/mL BSA, 50% glycerol. |
| Optimal buffer(10X) | 100 mM Tris-HCl (pH 8.5), 100 mM MgCl2, 1000 mM KCl,1 mg/mL BSA. |
| Unit Definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Assayed on | Lambda DNA |
| Optimal temperature | 37°C |
| Ligations | After 10-fold over digestion with enzyme 95% of the DNA fragments can be ligated and recut |
| Methylation Effects: | Not dam methylation-sensitive, not dcm methylation- sensitive. CpG methylation-sensitive. |
| Thermal Inactivation: | Enzyme is inactivated by incubation at 80°C for 20 minutes. |
| Campatible Ends | Sal I, Eco88 I, PspX I, Smo I, SgrD I. |
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