0
High enzyme concentration may result in star activity.
| Sources | Recombinant E. coli strain that carries the cloned salIR gene from Streptomyces albus. |
| Storage conditions | 10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/mL BSA, 50% glycerol. |
| Optimal buffer(10X) | 500 mM Tris-HCl (pH 7.5), 100 mM MgCl2, 1000 mM NaCl, 1 mg/mL BSA. |
| Unit Definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Assayed on | Lambda DNA |
| Methylation Effects: | Not dam methylation-sensitive, not dcm methylation-sensitive. CpG methylation-sensitive |
| Campatible Ends | Eco88 I, SgrD I, Smo I, Xho I |
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