At 37°C activity is 50% from maximum
Sources | Recombinant E. coli strain that carries the cloned stuIR gene from Streptomyces tubercidicus. |
Storage conditions | 10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM EDTA, 1 mM DTT, 0.2mg/ml BSA, 50% glycerol. |
Optimal buffer(10X) | 100 mM Tris-HCl (pH 7.5), 100 mM MgCl2, 1 mg/ml BSA. |
Unit Definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
Optimal temperature | 37°C |
Ligations | After 10-fold over digestion with enzyme 90% of the DNA fragments can be ligated and recut. |
Methylation Effects: | Not dam methylation-sensitive, Not CpG methylation-sensitive. dcm methylation-sensitive. |
Campatible Ends | Stu I ends are compatible with any blunt end |
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