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High enzyme concentration may result in star activity. Long incubation with BSA is not recmmenden due to star activity. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.?
| Sources | Recombinant E. coli strain that carries the cloned kpnIR gene from Klebsiella pneumoniae |
| Storage conditions | 10 mM Tris-HCl (pH 7.5 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/mL BSA, 50% glycerol. |
| Optimal buffer(10X) | 100 mM Tris-HCl (pH 7.5 at 37°C), 100 mM MgCl2, 0.2% Triton X-100, 1 mg/mL BSA. |
| Ligations | After 10-fold over digestion with enzyme 95% of the DNA fragments can be ligated and recut. |
| Methylation Effects: | Methylation sensitivity not tested |
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