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High enzyme concentration results in star activity
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Recognition sites | TAC↑GTA |
| Source | Bacillus stearothermophilus SN |
| Assayed on | T7 DNA |
| Optimal buffer | B?(10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) |
| Optimal temperature | 37°C |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 100 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at 20°C. |
| Ligations | After 5-fold overdigestion with enzyme about 70% of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 5 u.a. of enzyme for 16 hours at 37°C. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 80°C for 20 mins. |
| Campatible Ends | Eco105I, SnaBI |
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