To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.? Do not use BSA for long incubation.
Sources | Recombinant E. coli strain that carries the cloned nheIR gene from Neisseria mucosa. |
Storage conditions | 10 mM Tris-HCl (pH 8.0 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/mL BSA, 50% glycerol. |
Optimal buffer(10X) | 330 mM Tris-acetate (pH 7.9), 100 mM magnesium acetate, 660 mM potassium acetate, 1 mg/mL BSA. |
Unit Definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
Assayed on | Lambda DNA |
Methylation Effects: | Not dam methylation-sensitive, not dcm methylation- sensitive. CpG methylation-sensitive |
Campatible Ends | Eco130 I, Avr II, Bcu I, Spe I, Xba I, XmaJ I. |
温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知,且每位咨询者购买情况、提问时间等不同,为此以下回复仅对提问者3天内有效,其他网友仅供参考!若由此给您带来不便请多多谅解,谢谢!
服务热线
0771-3293894