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To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
| Sources | Recombinant E. coli strain that carries the cloned ncoIR gene from Nocardia corallina. |
| Storage conditions | 10 mM Tris-HCl (pH 7.5 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/mL BSA, 50% glycerol. |
| Optimal buffer(10X) | 330 mM Tris-acetate (pH 7.9), 100 mM magnesium acetate, 660 mM potassium acetate, 1 mg/mL BSA. |
| Ligations | After 10-fold over digestion with enzyme 95% of the DNA fragments can be ligated and recut. |
| Methylation Effects: | Not dam methylation-sensitive, not dcm methylation-sensitive. CpG methylation-sensitive. |
| Campatible Ends | Afl III, Psc I, Btg I, Pag I, BspLU11 I, Dsa I, Rca I, Eco130 I, Fat I. |
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