Sensitive to impurities present in some DNA preparations. For example, DNA purified by standard miniprep procedures is cleaved at lowe rates. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
Sources | Recombinant E. coli strain that carries the cloned ndeIR gene from Neisseria denitrificans |
Storage conditions | 10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/mL BSA, 50% glycerol. |
Optimal buffer(10X) | 500 mM Tris-HCl (pH 7.5), 100 mM MgCl2, 1000 mM NaCl, 1 mg/mL BSA. |
Ligations | After 10-fold over digestion with enzyme 95% of the DNA fragments can be ligated and recut. |
Campatible Ends | Asn I, Mae I, Tru9 I, Csp6 I, FspB I, Tru1 I, Vsp I. |
温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知,且每位咨询者购买情况、提问时间等不同,为此以下回复仅对提问者3天内有效,其他网友仅供参考!若由此给您带来不便请多多谅解,谢谢!
服务热线
0771-3293894