概述

Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5→3 polymerase activity and 3→5 exonuclease (proofreading) activity, but lacks 5→3 exonuclease activity of DNA polymerase I.

特点

• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides) Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffers

应用

DNA blunting by fill-in 5-overhangs Random-primed DNA labeling Labeling by fill-in 5-overhangs of dsDNA

技术规格