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M-MuLV Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase RNase H Minus and function in a wide range of temperature（42~55°C）.
First strand cDNA synthesis for RT-PCR and real-time RT-PCR Synthesis of cDNA for cloning and expression Generation of labeled cDNA probes for microarrays DNA labeling Analysis of RNA by primer extension
M-MuLV Reverse Transcriptase, 5× Reaction Buffer
| Storage buffer | 50 mM Tris-HCl (pH7.5), 0.1 M NaCl, 1 mM EDTA, 5 mM DTT, 0.1%（v/v）Triton X-100 and 50%（v/v）glycerol |
| Unit definition | One unit of the enzyme incorporates 1 nmol of dTMP into a polynucleotide fraction (adsorbed on DE-81) in 10 min at 37°C. |
| 5 x M-MuLV RT buffer | 250 mM Tris-HCl (pH8.3, 25°C), 250 mM KCl, 20 mM MgCl2, 50 mM DTT |
| inhibitor | Metal chelators, inorganic phosphate, pyrophosphate and polyamines |
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