Pfu DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5’→3’direction. Pfu DNA Polymerase also exhibits 3’→5’exonuclease (proofreading) activity that enables the polymerase to correct nucleotide incorporation errors. It has no 5’→3’exonuclease activity
High fidelity PCR Generation of PCR products for cloning and expression RT-PCR for cDNA cloning and expression Blunt-end PCR cloning
Pfu DNA Polymerase (native or recombinant, 2.5 U/μL), 10X Pfu Buffer with MgSO4, 10X Pfu Buffer, 25 mM MgSO4
Unit definition | One unit of the enzyme catalyzes the incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30 minutes at 72°C. |
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