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Pfu DNA polymerase 500U

价:
105.00
价:
¥105.00

号:B600003-0500

牌:生工

账期 货到付款

EA (预计4-5工作日到货)

工作时间

周一至周五:9:00-18:00

咨询电话

0771-3293894

在线咨询

客服 郭恒 蔡玉坤 曾宪飞 技术咨询

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产品描述

概述

Pfu DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5’→3’direction. Pfu DNA Polymerase also exhibits 3’→5’exonuclease (proofreading) activity that enables the polymerase to correct nucleotide incorporation errors. It has no 5’→3’exonuclease activity

特点

  • Eight times more accurate than Taq DNA polymerase Highly thermostable – remains 95% active after 2 hours incubation at 95°C Generates blunt-end PCR products Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)

应用

High fidelity PCR Generation of PCR products for cloning and expression RT-PCR for cDNA cloning and expression Blunt-end PCR cloning

组份

Pfu DNA Polymerase (native or recombinant, 2.5 U/μL), 10X Pfu Buffer with MgSO4, 10X Pfu Buffer, 25 mM MgSO4

技术规格

Unit definition One unit of the enzyme catalyzes the incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30 minutes at 72°C.

其它

储存:冷冻(-20℃)

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0771-3293894