概述

HotStart Taq DNA Polymerase is chemically modified, and suitable for hotstart PCR, because of chemically modified, so the activity of Taq enzyme was inhibited. The activity of the enzyme is completely inhibited at room temperature. But at 94°C, 10 min, this inhibition disappears, the enzyme restore vitality and can polymerized DNA，This method avoids the non-specific amplification due to primer nonspecific annealing or primer dimer at low temperature conditions.

特点

• This enzyme can also be used for the amplification of fluorescence quantitative PCR, and has a high efficiency of amplification.

应用

PCR amplification of complex template DNA. PCR amplification of complex template cDNA（RT-PCR）.Sequencing of single or double stranded DNA.site-directed mutagenesis. Real-Time PCR.

组份

HotStart Taq DNA Polymerase , 5X PCR Buffer（contain 10 mM MgCl2）

技术规格