概述

Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5’→3’synthesis of DNA, has no detectable 3’→5’exonuclease (proofreading) activity and possesses low 5’→3’exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3’-end of PCR products. Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter.

特点

• Generates PCR products with 3’-dA overhangs half life is more than 40 min at 95°C Incorporates modified nucleotides

应用

Routine PCR amplification of DNA fragments up to 5 kb High throughput PCR DNA labeling

组份

Taq DNA Polymerase 5 U/μL;10X Taq Buffer with KCl;10X Taq Buffer with (NH4)2SO4;25 mM MgCl2

技术规格