Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5’→3’synthesis of DNA, has no detectable 3’→5’exonuclease (proofreading) activity and possesses low 5’→3’exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3’-end of PCR products. Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter.
Routine PCR amplification of DNA fragments up to 5 kb High throughput PCR DNA labeling
Taq DNA Polymerase 5 U/μL;10X Taq Buffer with KCl;10X Taq Buffer with (NH4)2SO4;25 mM MgCl2
Unit definition | One unit of the enzyme catalyzes the incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30 min at 70°C. |
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