Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Analysis Note

Use 125 μl CelLytic M for 10⁶-10⁷ of suspended cells. For adherent cells, use 500-1,000 μL for a 100 mm plate; 200-400 μL for a 35 mm plate.

Features and Benefits

? Efficient: Up to 50% more efficient than freeze thaw, sonication and other products
? Non-denaturing: Does not interfere in downstream applications such immunoprecipitation, kinase and phosphatase assays, reporter gene assays and gel shift assays
? Convenient: Ready-to-use reagent requires no scraping from culture plates
? Fast: Rapid cell lysis at room temperature.

General description

CelLytic M is a proprietary detergent solution designed for efficient whole-cell protein extraction from cultured mammalian cells. It enables efficient and rapid cell lysis and solubilization of proteins for both suspension and adherent cells. Lysates can be used in many downstream applications without removing the CelLytic M such as reporter gene assays, Western blots/immunoprecipitation, electrophoretic mobility shift assays, phosphatase assays and kinase asssays.

Quantity

CelLytic M reagent efficiency for protein extraction has been tested on, but not limited to, HeLa, CHO, COS, HL-60, Jurkat, A431, PC-12, and Bovine Aorta Endothelial Cells (BAEC).

法律信息

CelLytic is a trademark of Sigma-Aldrich Co. LLC