Popular Documents: Specification Sheet (PDF)
Human cathepsin B (GenBank Accession No. NM_001908), CD33 signal peptide(amino acids 1-16) + CathepsinB (amino acids 18-339), with C-terminal HIS tag, MW = 43 kDa, expressed in FreeStyle 293-F cells.
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C
Formulated in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole.
Active human cathepsin B is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active human cathepsin B has been used in a study to assess maturation through proenzyme activation and proteolytic processing. Active human cathepsin B has also been used in a study to prepare a catalytically active version from its precursor expressed in Escherichia coli in the form of inclusion bodies.
FreeStyle 为以下机构的商标: Invitrogen Corp.
recombinant | expressed in FreeStyle? 293-F cells |
assay | ≥90% (SDS-PAGE) |
form | aqueous solution |
mol wt | mol wt 43 kDa |
NCBI accession no. | NM_001908 |
shipped in | dry ice |
storage temp. | ?70°C |
Gene Information | human ... CTSB(1508) |
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