MDL number MFCD00165844
Popular Documents: Bulletin (PDF) | Specification Sheet (PDF)
SigmaMarkers are designed for use with Laemmli SDS-PAGE, the PhastSystem (Pharmacia) and Schagger and von Jagow SDS-PAGE (Tricine). SigmaMarkers cover the range of molecular weights common to most proteins or their subunits. The wide, high and low range markers are lyophilized with sample buffer so that they are ready-to-use after reconstitution with deionized water. The markers are formulated to yield a distribution of well-defined bands of approximately equal intensity after electrophoresis and Coomassie blue staining.
Protein (mol. wt.)
Glyceraldehyde-3-phosphat
Ovalbumin, chicken egg (45,000)
Glutamic dehydrogenase, bovine liver (55,000)
Albumin, bovine serum (66,000)
Phosphorylase B, rabbit muscle (97,000)
β-Galactosidase, E. coli (116,000)
Myosin, porcine heart (200,000)
Will yield bands for the following seven proteins from the SigmaMarker list with Coomassie Blue staining.
Reconstitution with 100 μl of deionized water results in a solution containing 2-3.5 mg of protein per ml of 62 mM Tris-HCl, pH 6.8, 1 mM EDTA, 4% sucrose, 10 mM TCEP, 2% SDS, and 0.01% bromophenol blue.
SigmaMarker 为以下机构的商标: Sigma-Aldrich Co. LLC
form | lyophilized powder |
usage | sufficient for 10-20 large gels (16 × 18 cm) |
sufficient for 20-30 mini-gels (10 × 10 cm) | |
1 vial sufficient for 50-100 PhastGels | |
mol wt | mol wt 36,000-200,000 Da |
storage temp. | 2-8°C |
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