Components

2X reaction buffer containing dATP, dCTP, dGTP, dTTP, magnesium chloride, reverse transcriptase, RNase inhibitor protein, hot-start DNA polymerase, inert blue qPCR dye, and stabilizers

packaging:
500 reactions* = 5 X 1 mL tubes
*number of reactions based on a 20uL volume

Features and Benefits

• 仅需33分钟即可获得检测结果
• 高效和高灵敏度的实时PCR结果
• 极少/无需优化
  
  

  General description

  KiCqStart One-Step Probe RT-qPCR ReadyMix is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan^® 5’-hydrolysis probes on real-time PCR systems (refer to instrument compatibility section to select the appropriate reagent for your machine). First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets as well as high throughput gene-expression studies. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times.
  
  KiCqStart One-Step Probe RT-qPCR ReadyMix contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries. The reverse transcriptase is an engineered M-MLV with reduced RNase H activity and improved activity and stability at higher temperatures. The use of higher temperatures (50 to 55°C) for the first-strand step of one-step RT-qPCR provides higher specificity for primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis.
  
  KiCqStart One-Step Probe RT-qPCR ReadyMix is highly resistant to PCR inhibitors. A key component of the ReadyMix is an ultra-pure, highly processive, thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This provides an extremely stringent automatic hot-start that minimizes the potential for primer-dimer and other non-specific PCR artifacts. The light blue color of the inert dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. The dye does not interfere with qPCR performance or affect the stability of the product.

  Application

  Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart One-Step Probe RT-qPCR ReadyMix does not contain an internal reference dye. Compatible instruments include:
  ? Bio-Rad^® CFX384^?
  ? Bio-Rad CFX96^?
  ? Bio-Rad MiniOpticon^?
  ? Bio-Rad/MJ Chromo4^?
  ? Bio-Rad/MJ Opticon 2
  ? Bio-Rad/MJ Opticon^?
  ? Cepheid SmartCycler^®
  ? Eppendorf Mastercycler^® ep realplex
  ? Eppendorf Mastercycler ep realplex2 s
  ? Illumina Eco qPCR
  ? Qiagen/Corbett Rotor-Gene^® 3000
  ? Qiagen/Corbett Rotor-Gene 6000? Qiagen/Corbett Rotor-Gene Q
  ? Roche LightCycler^® 480
  
  

  法律信息

  Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.

  CFX384 is a trademark of Bio-Rad Laboratories, Inc.

  CFX96 is a trademark of Bio-Rad Laboratories, Inc.

  Chromo4 is a trademark of Bio-Rad Laboratories, Inc.

  KiCqStart is a registered trademark used under license.

  LightCycler is a registered trademark of Roche

  Mastercycler is a registered trademark of Eppendorf AG

  MiniOpticon is a trademark of Bio-Rad Laboratories, Inc.

  Opticon is a trademark of Bio-Rad Laboratories, Inc.

  ReadyMix is a trademark of Sigma-Aldrich Co. LLC

  Rotor-Gene is a registered trademark of Qiagen Group

  SmartCycler is a registered trademark of Cepheid, Inc.

  TaqMan is a registered trademark of Roche Molecular Systems, Inc.