Components

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, ROX reference dye, inert blue qPCR dye, and stabilizers

Features and Benefits

• 仅需33分钟即可获得检测结果
• 高效和高灵敏度的实时PCR结果
• 极少/无需优化
  
  

  General description

  KiCqStart Probe qPCR ReadyMix is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan^® hydrolysis probes. KiCqStart Probe qPCR ReadyMix is provided as a 2X concentrated ready-to-use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue color of an inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.
  
  A key component of KiCqStart Probe qPCR ReadyMix is an ultra-pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA§. KiCqStart Probe qPCR ReadyMix is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples. The enzyme in KiCqStart Probe qPCR ReadyMix is combined with high avidity monoclonal antibodies to provide a stringent automatic hot-start that allows reaction assembly, and temporary storage, at room temperature prior to PCR amplification.

  Application

  不同的实时PCR体系采用不同的策略来标准化荧光信号和校正孔与孔之间的光学差异。选择与您的qPCR试剂匹配的仪器至关重要。KiCqStart One-Step Probe RT-qPCR ReadyMix不含内参染料。兼容仪器包括：
  

  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7800
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems 7900 HT
  • Applied Biosystems StepOnePlus^?
  • Applied Biosystems StepOne^?
    
    

    法律信息

    StepOne 为以下机构的商标： Applera Corporation or its subsidiaries in the US and/or certain other countries

    StepOnePlus 为以下机构的商标： Applera Corporation or its subsidiaries in the US and/or certain other countries