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2X reaction buffer containing optimized concentrations of MgCl2, dNTPs, (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, ROX Reference Dye (for 580-585 nm excitation), and stabilizers.
packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume
Instrument Compatibility:
Different real-time PCR systems employ different strategies for the normalization of fluorescent signals and correction of the well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix, with ROX is available for the following platforms:
? Applied Biosystems 5700
? Applied Biosystems 7000
? Applied Biosystems 7300
? Applied Biosystems 7700
? Applied Biosystems 7900
? Applied Biosystems 7900 HT Fast
? Applied Biosystems 7900 HT
? Applied Biosystems StepOnePlus?
? Applied Biosystems StepOne?
PCR applications:
? Gene expression
? DNA quantitation
? CHiP
? Assay results in as little as 33 minutes
? Highly efficient and sensitive real-time PCR results
? Little/no optimization required
KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers and template, for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR.
Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step. The optimized formulation includes SYBR Green I dye, an antibody-mediated Hot-Start Taq DNA polymerase, dNPTs, MgCl2 and proprietary buffers and stabilizers.
Storage Conditions:
KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.
KiCqStart is a registered trademark used under license.
ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
form | liquid |
feature | hotstart |
storage condition | protect from light |
color | colorless |
compatibility | for use with ABI 5700 |
for use with ABI 7000 | |
for use with ABI 7300 | |
for use with ABI 7700 | |
for use with ABI 7900 Fast | |
for use with ABI 7900 HT | |
for use with ABI 7900 | |
for use with ABI StepOne | |
for use with ABI StepOnePlus | |
shipped in | dry ice |
storage temp. | ?20°C |
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