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2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, and stabilizers
packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume
PCR applications:
? Gene expression
? DNA quantitation
? CHiP
Instrument Compatibility:
Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix does not contain an internal reference dye. Compatible instruments include:
? Bio-Rad® CFX384?
? Bio-Rad CFX96?
? Bio-Rad MiniOpticon?
? Bio-Rad/MJ Chromo4?
? Bio-Rad/MJ Opticon 2
? Bio-Rad/MJ Opticon?
? Cepheid SmartCycler®
? Eppendorf Mastercycler® ep realplex
? Eppendorf Mastercycler ep realplex2 s
? Illumina Eco qPCR
? Qiagen/Corbett Rotor-Gene® 3000
? Qiagen/Corbett Rotor-Gene 6000
? Qiagen/Corbett Rotor-Gene Q
? Roche LightCycler® 480
? Assay results in as little as 33 minutes
? Highly efficient and sensitive real-time PCR results
? Little/no optimization required
KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers and template, for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR.
Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step. The optimized formulation includes SYBR Green I dye, an antibody-mediated Hot-Start Taq DNA polymerase, dNPTs, MgCl2 and proprietary buffers and stabilizers.
Storage Conditions:
KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.
Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.
CFX384 is a trademark of Bio-Rad Laboratories, Inc.
CFX96 is a trademark of Bio-Rad Laboratories, Inc.
Chromo4 is a trademark of Bio-Rad Laboratories, Inc.
Eppendorf is a registered trademark of Eppendorf AG
KiCqStart is a registered trademark used under license.
LightCycler is a registered trademark of Roche
Mastercycler is a registered trademark of Eppendorf AG
MiniOpticon is a trademark of Bio-Rad Laboratories, Inc.
Opticon is a trademark of Bio-Rad Laboratories, Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Rotor-Gene is a registered trademark of Qiagen Group
SYBR is a registered trademark of Life Technologies
SmartCycler is a registered trademark of Cepheid, Inc.
form | liquid |
feature | hotstart |
storage condition | protect from light |
color | colorless |
compatibility | for use with Bio-Rad CFX384 |
for use with Bio-Rad CFX96 | |
for use with Bio-Rad MJ Chromo4 | |
for use with Bio-Rad MJ Opticon 2 | |
for use with Bio-Rad MJ Opticon Cepheid SmartCycler | |
for use with Bio-Rad MiniOpticon | |
for use with Bio-Rad MyiQ | |
for use with Eppendorf® Mastercycler ep realplex2 s | |
for use with Eppendorf® Mastercycler ep realplex | |
for use with Illumina Eco qPCR | |
for use with Qiagen Corbett Rotor-Gene 3000 | |
for use with Qiagen Corbett Rotor-Gene 6000 | |
for use with Qiagen Corbett Rotor-Gene Q | |
for use with Roche LightCycler 480 | |
shipped in | dry ice |
storage temp. | ?20°C |
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