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The FLAG Purification Kit utilizes CelLytic? Reagents for rapid and efficient cell lysis and protein extraction and ANTI-FLAG® M2 affinity gel for affinity purification of active FLAG fusion proteins.
Browse additional application references in our FLAG® Literature portal.
? Includes ready to use reagents, columns, and a detailed protocol for affinity purification of FLAG fusion proteins.
? ANTI-FLAG M2 Affinity Gel allows efficient binding of FLAG fusion proteins without the need for preliminary steps and calibrations.
? Two alternatives for efficient elution conditions (by acidic conditions or by competition with FLAG peptide).
Kit contains CelLytic? M for cell lysis and protein extraction from Mammalian cells.
Sufficient for 3-5 uses of 1-ml affinity purification column.
CelLytic is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
shipped in | wet ice |
storage temp. | ?20°C |
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