MDL number MFCD00165986
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TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.
TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions. Autoclaving is not recommended without testing for changes in properties. TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures. Polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid. They may also reduce the activity of many preservatives. Aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate.
50, 100 mL in poly bottle
TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.
TWEEN 为以下机构的注册商标: Croda International PLC
grade | for molecular biology |
description | non-ionic |
form | viscous liquid |
mol wt | mol wt ~1228 |
mol wt ~1228 | |
composition | lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids) |
refractive index | n |
CMC | 60 mg/L |
density | 1.095 g/mL at 25 °C(lit.) |
suitability | suitable for molecular biology |
foreign activity | Endonuclease-exonuclease and RNase, none detected |
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