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Popular Documents: Bulletin (PDF) | Specification Sheet (PDF)
The HIS-Select Nickel Magnetic Agarose Beads are designed for use in automated and small-scale affinity capture (molecular pull-down) purifications of histidine-tagged protein while exhibiting low non-specific binding of other proteins. The beads can be used to puriy proteins under native and denaturing conditions.
HIS-Select Magnetic Agarose Beads consist of paramagnetic, immobilized metal-ion affinity chromatography (IMAC) resin that contain a proprietary quadridentate chelate, which is bound with nickel and covalently attached through a non-charged, hydrophilic linker to magnetic beaded agarose. The magnetic properties of the beads aid in manipulations, such as repetitive washings, and recovery of the protein bound beads. This leads to greater experimental reproducibility and more accurate quantitation of the proteins of interest.
HIS-Select 为以下机构的注册商标： Sigma-Aldrich Co. LLC
| form | (Supplied as a 50% slurry suspension in 30% Ethanol.) |
| shelf life | 2 yr (Unopened product) |
| matrix | 6% beaded magnetic agarose |
| capacity | ≥15 mg/mL binding capacity |
| storage temp. | 2-8°C |
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